TOP 2-FDCK HCL SECRETS

The lab states fixation will induce the dyes to leak in the cells. I feel the assumption is the fact that fixation may possibly hurt cell membranes. I did locate a paper which describes fixation working with methanol or acetic acid, following staining which has a DCFDA derivative. DCFDA will be the reagent that stains ROS while in the kit ab113851,